Table of Contents

• Section 1. Frank's Story
• 1: Recap the story
• Section 2. How Could We Measure the Infection?
• 2: Count 'em all?
• 3: Direct count
• 4: Light scatter (using spec)
• 5: The dilemma of the dead cell
• 6: Viable plate count
• 7: Comparison of methods
• Section 3. How to Do a Viable Plate Count
• 8: Why we need to dilute
• 9: The dilution factor
• 10: Overall dilution
• 11: Interlude: Diluting Cold Press Coffee
• 12: How to scale back up
• 13: Why we plate more than one dilution
• 14: Picking the plate count
• Section 4. Practice Problems
• 15: Putting it all together
• 16: More dilution problems
• 17: Quickies
• Section 5. Back to Poor Frank
• 18: Is he dead yet?

Putting it all together

Here is a chance for you to put all of the steps together, using viable plate count and serial dilution to quantify a bacterial population...