Table of Contents

• Prequiz
• 1: Three thieves and a beaded necklace
• 2: ... and its moral
• 3: A real plasmid
• 4: Practice with restriction sites
• 5: What's wrong with these gels?
• 6: Playing with plasmids
• 7: Other weirdnesses
• 8: Review

Playing with plasmids

This first applet is mainly for fun. You can click on the applet to add restriction sites for A and B, then run the gel to see what it looks like.

 

Now that you have some experience under your belt, see if you can find the missing restriction site in the following applet:

 

Finally, can you figure out what plasmid could produce the following gel? (There are multiple correct answers -- for example, you could add the second A site either clockwise or anti-clockwise. Just try to find one possibility).