Table of Contents

• Prequiz
• 1: Three thieves and a beaded necklace
• 2: And the moral is?
• 3: A real plasmid
• 4: Practice with restriction sites
• 5: What's wrong with these gels?
• 6: Playing with plasmids
• 7: Other weird things
• 8: Summary

Playing with plasmids

This first applet is mainly for fun. You can click on the applet to add restriction sites for A and B, then run the gel to see what it looks like:

Now that you have some experience under your belt, see if you can find the missing restriction site in the following applet:

Finally, can you figure out what plasmid could produce the following gel? (There are multiple correct answers -- for example, you could add the second A site either clockwise or anti-clockwise. Just try to find one possibility).