Table of Contents

• Prequiz
• 1: Three thieves and a beaded necklace
• 2: And the moral is?
• 3: A real plasmid
• 4: Practice with restriction sites
• 5: What's wrong with these gels?
• 6: Playing with plasmids
• 7: Other weird things
• 8: Summary

Practice with restriction sites

Ready for something harder? In this applet, you are asked to predict what the gel will look like, given that you know the map of the plasmid.

When you get all three lanes filled out, you should notice a pattern. As you use more enzymes, you get more pieces, but those pieces are generally shorter. Translated into gel-speak, that means lanes with more enzymes have more bands, and they’re clustered close to the end (rather than beginning) of the lane.