Table of Contents

• Section 1. How to Do a Viable Plate Count
• 1: Why we need to dilute
• 2: Setting the stage: Diluting Cold Press Coffee
• 3: Working out the dilution
• 4: Overall dilution
• 5: Design a dilution!
• 6: How to scale back up
• 7: Count a plate!
• 8: Why plate more than one dilution?
• 9: Picking the plate to count
• Section 2. Practice Problems
• 10: Putting it all together
• 11: Quickies
• 12: Is the water safe?
• Section 3. Review
• 13: Summary

Count a plate!

Once again, let's take a breather from the theory and practice. This is one of the do-it-in-your-sleep kind, and it has two steps:

1. Look at the dilution scheme to determine the total dilution, and
2. Look at the plate and scale up to determine the total CFUs per mL.